POU2AF1 antibody (AA 150-256)

Catalog No. ABIN8123906

This Mouse Monoclonal antibody specifically detects POU2AF1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for POU2AF1 antibody (AA 150-256) (ABIN8123906)

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This POU2AF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: AB1494

Product Details anti-POU2AF1 Antibody

Binding Specificity: AA 150-256

Purpose: BOB.1 mouse mAb

Specificity: The antibody can specifically recognize human BOB. 1 protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 150 - 256 of the human BOB. 1 protein.

Isotype: IgG

Application Details

Application Notes: IHC, 1:200-1:1000 | WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Alternative Name: BOB.1

Background:

Synonyms: B cell Oct binding protein 1, B cell specific coactivator OBF 1, B cell specific coactivator OBF1, B-cell-specific coactivator OBF-1, BOB 1, BOB-1, OBF 1, OBF1, OBF1_HUMAN, OCA B, OCA-B, OCAB, OCT binding factor 1, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2 associating factor 1, POU domain class 2-associating factor 1, Pou2af1

Background: BOB. 1 is a transcription activating factor necessary for the formation of follicular germinal center and the production of immunoglobulin. It works together with Oct-2 and is highly expressed in granulocytes of spleen and peripheral blood. It is mainly used for the typing of Hodgkin's lymphoma, mainly nodular lymphocytes. Hodgkin's lymphoma is positive and classical Hodgkin's lymphoma is negative.

Gene Name: POU2AF1

Protein Name: BOB. 1

Gene ID: 5450

UniProt: Q16633