OCT4 antibody (AA 50-150)

Catalog No. ABIN8123908

The Mouse Monoclonal anti-OCT4 antibody (Clone AB2411) (ABIN8123908) specifically detects OCT4 in WB, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for OCT4 antibody (AA 50-150) (ABIN8123908)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: AB2411

Product Details anti-OCT4 Antibody

Binding Specificity: AA 50-150

Purpose: Oct-4 mouse mAb

Specificity: This antibody detects endogenous levels of Oct-4 protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 50 - 150 of the human 45934 protein.

Isotype: IgG

Application Details

Application Notes: WB, 1:500-1:2000 | IF, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: Oct-4

Background:

Synonyms: POU5F1,OCT3,OCT4,OTF3,POU domain,class 5, transcription factor 1,Octamer-binding protein 3,Oct-3,Octamer-binding protein 4,Oct-4,Octamer-binding transcription factor 3,OTF-3

Background: This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Gene Name: POU5F1

Protein Name: Oct-2 protein

Gene ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance