RAD21 antibody

Catalog No. ABIN8124221

This Rabbit Monoclonal antibody specifically detects RAD21 in WB, IF, ELISA, IHC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RAD21 antibody (ABIN8124221)

Target: RAD21 (RAD21 Homolog (RAD21))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This RAD21 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: AB1715

Product Details anti-RAD21 Antibody

Purpose: Rad21 Rabbit mAb

Specificity: This antibody detects endogenous levels of Rad21 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for RAD21

Target: RAD21 (RAD21 Homolog (RAD21))

Alternative Name: Rad21

Background:

Synonyms: RAD21, HR21, KIAA0078, NXP1, Double-strand-break repair protein rad21 homolog, hHR21, Nuclear matrix protein 1, NXP-1, SCC1 homolog

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008],

Gene Name: RAD21

Protein Name: Rad21

Gene ID: 5885

UniProt: O60216

Pathways: Positive Regulation of Endopeptidase Activity