Sonic Hedgehog antibody

Catalog No. ABIN8124853

The Rabbit Polyclonal anti-Sonic Hedgehog antibody (ABIN8124853) specifically detects Sonic Hedgehog in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Sonic Hedgehog antibody (ABIN8124853)

Target: Sonic Hedgehog (SHH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Sonic Hedgehog Antibody

Purpose: Shh Antibody

Specificity: This antibody detects endogenous levels of human Shh

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Shh

Isotype: IgG

Application Details

Application Notes: IHC 1:50-200, WB 1:500-2000. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Alternative Name: Shh

Background:

Synonyms: Sonic hedgehog protein (SHH, HHG-1) [Cleaved into: Sonic hedgehog protein N-product, Sonic hedgehog protein C-product]

Background: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a d

Gene Name: SHH

Protein Name: Shh

Gene ID: 6469

UniProt: Q15465

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development