T-Bet antibody

Catalog No. ABIN8125078

The Mouse Monoclonal anti-T-Bet antibody (Clone AB0815) (ABIN8125078) specifically detects T-Bet in WB, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for T-Bet antibody (ABIN8125078)

Target: T-Bet

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: AB0815

Product Details anti-T-Bet Antibody

Purpose: T-bet mouse mAb

Specificity: This antibody detects endogenous levels of T-bet protein.

Purification: The antibody was purified by protein G affinity chromatography.

Immunogen: A synthetic peptide corresponding to the amino acid region 100-200 of the human T-bet protein.

Isotype: IgG

Application Details

Application Notes: WB, 1:500-1:2000 | IF, 1:100-400 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for T-Bet

Target: T-Bet

Background:

Synonyms: T-box transcription factor TBX21 (T-box protein 21) (T-cell-specific T-box transcription factor T-bet) (Transcription factor TBLYM)

Background: T-box 21(TBX21) Homo sapiens This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells. [provided by RefSeq, Jul 2008],

Gene Name: TBX21

Protein Name: T-bet

Gene ID: 30009

UniProt: Q9UL17