Topoisomerase I antibody

Catalog No. ABIN8125244

This Rabbit Polyclonal antibody specifically detects Topoisomerase I in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Topoisomerase I antibody (ABIN8125244)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Topoisomerase I antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Topoisomerase I Antibody

Purpose: Topo I Antibody

Specificity: Topo I Polyclonal Antibody detects endogenous levels of Topo I protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Topo I.

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Alternative Name: Topo I

Background:

Synonyms: TOP1, DNA topoisomerase 1, DNA topoisomerase I

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008],

Gene Name: TOP1

Protein Name: DNA topoisomerase 1

Gene ID: 7150

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance