ULK1 antibody (pSer757)

Catalog No. ABIN8125603

The Rabbit Monoclonal anti-ULK1 antibody (Clone AB2194) (ABIN8125603) specifically detects ULK1 in WB, ELISA, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ULK1 antibody (pSer757) (ABIN8125603)

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ULK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2194

Product Details anti-ULK1 Antibody

Binding Specificity: pSer757

Purpose: ULK1 (Phospho Ser757) Rabbit mAb

Specificity: The antibody detects endogenous levels of ULK1 protein only when phosphorylated at Ser757. The antibody does not cross-react with ULK1 phosphorylated at other sites.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for ULK1

Target: ULK1 (Unc-51 Like Kinase 1 (ULK1))

Alternative Name: ULK1

Background:

Synonyms: ULK1, KIAA0722, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, ATG1, hATG1, Unc-51-like kinase 1

Background: catalytic activity:ATP + a protein = ADP + a phosphoprotein. , function:Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells. , similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. , similarity:Contains 1 protein kinase domain. , subunit:Interacts with GABARAP and GABARAPL2. , tissue specificity:Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. ,

Gene Name: ULK1

Protein Name: ULK1 (Phospho Ser757)

Gene ID: 8408

UniProt: O75385

Pathways: Regulation of Cell Size, Autophagy