WT1 antibody (AA 310-390)

Catalog No. ABIN8125747

The Rabbit Polyclonal anti-WT1 antibody (ABIN8125747) specifically detects WT1 in WB and ELISA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for WT1 antibody (AA 310-390) (ABIN8125747)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-WT1 Antibody

Binding Specificity: AA 310-390

Purpose: WT1 Antibody

Specificity: WT1 Polyclonal Antibody detects endogenous levels of protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human protein . at AA range: 310-390

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000 ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS containing 50 % glycerol, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1

Background:

Background: This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq, Mar 2015],

Gene Name: WT1

Protein Name: Wilms tumor protein (WT33)

Gene ID: 7490

UniProt: P19544

Pathways: Tube Formation