XBP1 antibody

Catalog No. ABIN8125764

The Rabbit Monoclonal anti-XBP1 antibody is suitable to detect XBP1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and IP.

Quick Overview for XBP1 antibody (ABIN8125764)

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This XBP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB1852

Product Details anti-XBP1 Antibody

Purpose: XBP1 Rabbit mAb

Specificity: This antibody detects endogenous levels of XBP1 protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:1000-1:5000 | IHC, 1:200-1:1000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for XBP1

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: XBP1

Background:

Synonyms: XBP1, TREB5, XBP2, X-box-binding protein 1, XBP-1, Tax-responsive element-binding protein 5

Background: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER) , the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1) . The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1 (S) , which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1 (U) , is constitutively e

Gene Name: >>Protein processing in endoplasmic reticulum, >>Non-alcoholic fatty liver disease, >>Alzheimer disease, >>Parkinson disease, >>Amyotrophic lateral sclerosis, >>Spinocerebellar ataxia, >>Pathways of neurodegeneration-multiple diseases, >>Lipid and atherosclerosis

Protein Name: XBP1

Gene ID: 7494

UniProt: P17861

Pathways: ER-Nucleus Signaling, Unfolded Protein Response