XIAP antibody

Catalog No. ABIN8125765

The Rabbit Monoclonal anti-XIAP antibody (Clone AB2137) (ABIN8125765) specifically detects XIAP in WB, ELISA, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for XIAP antibody (ABIN8125765)

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This XIAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: AB2137

Product Details anti-XIAP Antibody

Purpose: XIAP Rabbit mAb

Specificity: This antibody detects endogenous levels of XIAP protein.

Purification: The antibody was purified by protein A affinity chromatography.

Immunogen: Recombinant protein (or fragment).

Isotype: IgG

Application Details

Application Notes: WB, 1:2000-1:10000 | IF, 1:200-1:1000 | IP, 0. 5 μg-4 μg antibody for 200 μg-400 μg extracts of whole cells. | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The antibody is provided in liquid form in phosphate - buffered saline with 50 % glycerol, 0.05 % BSA, and 0.05 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at-20°C. Avoid freeze / thaw cycles.

Target Details for XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Alternative Name: XIAP

Background:

Synonyms: XIAP, API3, BIRC4, IAP3, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4, IAP-like protein, ILP, hILP, Inhibitor of apoptosis protein 3, IAP-3, hIAP-3, hIAP3, X-linked inhibitor of apoptosis protein, X-linked I

Background: This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11. [provided by RefSeq, Feb 2011],

Gene Name: XIAP

Protein Name: XIAP

Gene ID: 331

UniProt: P98170

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Transition Metal Ion Homeostasis