KARS antibody (AA 301-400)

Catalog No. ABIN762821

The Rabbit Polyclonal anti-KARS antibody has been validated for WB, ELISA, IF (p), IF (cc), IHC (p) and IHC (fro). It is suitable to detect KARS in samples from Human.

Quick Overview for KARS antibody (AA 301-400) (ABIN762821)

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KARS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-KARS Antibody

Binding Specificity: AA 301-400

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human KARS2/Lysyl tRNA syntase

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for KARS

Target: KARS (Lysyl-tRNA Synthetase (KARS))

Alternative Name: Lysyl Trna Synthetase

Background:

Synonyms: KARS 2, KARS, KARS2, KIAA0070, KRS, Lysine tRNA ligase, Lysine--tRNA ligase, LysRS, Lysyl-tRNA synthetase, SYK_HUMAN.

Background: Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.

Gene ID: 6850