Angiopoietin 1 antibody (Internal Region)

Catalog No. ABIN768552

This anti-Angiopoietin 1 antibody is a Goat Polyclonal antibody detecting Angiopoietin 1 in WB, ELISA, FACS and IF. Suitable for Human.

Quick Overview for Angiopoietin 1 antibody (Internal Region) (ABIN768552)

Target: Angiopoietin 1 (ANGPT1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Angiopoietin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-Angiopoietin 1 Antibody

Binding Specificity: Internal Region

Purpose: ANGPT1

Sequence: PDFSSQKLQH

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PDFSSQKLQH, from the internal region of the protein sequence according to NP_001137.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected HEK293 transiently expressing full-length Human ANGPT1 (myc and DYKDDDDK tagged), a band of approx. 48 kDa was observed. No bands were observed in mock-transfected HEK293 and the 48 kDa plus a 75 kDa band was observed using anti

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the plasma membranes of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentr

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Angiopoietin 1

Target: Angiopoietin 1 (ANGPT1)

Alternative Name: ANGPT1

Background: ANGPT1, angiopoietin 1, AGP1, AGPT, ANG1

Molecular Weight: 48kDa

Gene ID: 284, 11600, 89807

NCBI Accession: NP_001137

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process