DNAJB9 antibody (AA 61-75)

Catalog No. ABIN768557

Product Details anti-DNAJB9 Antibody

Target: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Binding Specificity: AA 61-75

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DNAJB9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: DNAJB9 (aa61-75)

Sequence: PDAEAKFREI AEAYE

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-PDAEAKFREIAEAYE, from the internal regoin of the protein sequence according to NP_036460.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26-27 kDa band observed in Human Liver and Kidney lysates and approx.28 kDa in Mouse Liver lysates and in preliminary testing of Rat Liver lysate (calculated MW of 25.5 kDa according to Human NP_036460.1 and 25.6 kDa according to Mouse

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. R

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DNAJB9

Target: DNAJB9 (Microvascular Endothelial Differentiation Gene 1 Protein (DNAJB9))

Alternative Name: DNAJB9 (DNAJB9 Products)

Synonyms: ERdj4 antibody, MDG-1 antibody, MDG1 antibody, MST049 antibody, MSTP049 antibody, AA408011 antibody, AA673251 antibody, AA673481 antibody, AW556981 antibody, Mdg1 antibody, mDj7 antibody, DnaJ heat shock protein family (Hsp40) member B9 antibody, DNAJB9 antibody, Dnajb9 antibody

Background: DNAJB9, DnaJ (Hsp40) homolog, subfamily B, member 9, DKFZp564F1862, ERdj4, MDG1, MST049, MSTP049, dnaJ homolog subfamily B member 9, endoplasmic reticulum DnaJ homolog 4, mdg-1, microvascular endothelial differentiation gene 1 protein

Molecular Weight: 25.5kDa according to NP_036460.1

Gene ID: 4189

NCBI Accession: NP_036460

Pathways: ER-Nucleus Signaling