AKAP12 antibody (Internal Region)

Catalog No. ABIN768562

This Goat Polyclonal antibody specifically detects AKAP12 in ELISA. It exhibits reactivity toward Mouse.

Quick Overview for AKAP12 antibody (Internal Region) (ABIN768562)

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This AKAP12 antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-AKAP12 Antibody

Binding Specificity: Internal Region

Purpose: AKAP12 (mouse)

Sequence: SPDTNGPKLT EEGD

Specificity: This antibody is expected to recognize isoform 1 (NP_005091.2) only.

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SPDTNGPKLTEEGD, from the internal region of the protein sequence according to NP_112462.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary experiments in lysates of cell lines NIH3T3 and of Mouse and Rat Brain and Testis gave no specific signal but low background (at antibody concentration up to 2 μg/mL). We would appreciate any feedback from people in the field - h

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AKAP12

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Alternative Name: Akap12

Background: Akap12, A kinase (PRKA) anchor protein (gravin) 12, AI317366, SSeCKS, Srcs5, Tsga12, A-kinase anchor protein 12, AKAP-12, gercelin, germ cell lineage protein gercelin, src-suppressed C kinase substrate, testis specific gene A12, v-src suppressed transcri

Gene ID: 83397, 83425

NCBI Accession: NP_112462

Pathways: cAMP Metabolic Process