SOD2 antibody (AA 119-130)

Catalog No. ABIN768566

The Goat Polyclonal anti-SOD2 antibody has been validated for WB, IHC and ELISA. It is suitable to detect SOD2 in samples from Human, Rat, Mouse and Pig.

Quick Overview for SOD2 antibody (AA 119-130) (ABIN768566)

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Reactivity: Human, Rat, Mouse, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This SOD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SOD2 Antibody

Binding Specificity: AA 119-130

Purpose: MNSOD (aa119-130)

Sequence: EAIKRDFGSF DK

Specificity: NB: The immunizing peptide represents the acetylation site including K122 according to isoform A. This antibody is expected to recognize both reported isoforms (NP_000627.2, NP_001019637.1) . Reported variants represent identical protein: NP_000627.2, NP_

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EAIKRDFGSFDK, from the internal region of the protein sequence according to NP_000627.2, NP_001019637.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5 μg/mL.

Western Blot: Approx 24 kDa band observed in Human Brain (cerebellum) lysates and in Mouse and Rat Brain and Spinal Cord lysates, while approx 26 kDa band was observed in lysates of cell lines HeLa, HepG2, HEK293 and NIH3T3 (calculated MW of 24.8 kDa accor

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SOD2

Target: SOD2 (Superoxide Dismutase 2, Mitochondrial (SOD2))

Alternative Name: SOD2

Background: SOD2, superoxide dismutase 2, mitochondrial, RP1-56L9.2, IPOB, MNSOD, MVCD6, Mn superoxide dismutase, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase [Mn], mitochondrial

Molecular Weight: 24.8kDa according to Human NP_000627.2 and 24.6kDa according to Mouse NP_038699.2

Gene ID: 6648, 20656, 24787

NCBI Accession: NP_000627, NP_001019637

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Negative Regulation of intrinsic apoptotic Signaling