CHRNA5 antibody (Internal Region)
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- Target See all CHRNA5 Antibodies
- CHRNA5 (Cholinergic Receptor, Nicotinic, alpha 5 (Neuronal) (CHRNA5))
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Binding Specificity
- Internal Region
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This CHRNA5 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purpose
- CHRNA5
- Sequence
- HVDRYFTQKE ET
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-HVDRYFTQKEET, from the internal region of the protein sequence according to NP_000736.2.
- Isotype
- IgG
- Top Product
- Discover our top product CHRNA5 Primary Antibody
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Colon (submucosal plexus). Recommended concentration: 3.75 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line KELLY (calculated MW of 53.1 kDa according to NP_000736.2). Recommended concentration: 1-3 μg/mL. Primary incubation was 1 hour.
Peptide ELISA: antibody detection limit dilution 1:32000.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- CHRNA5 (Cholinergic Receptor, Nicotinic, alpha 5 (Neuronal) (CHRNA5))
- Alternative Name
- CHRNA5 (CHRNA5 Products)
- Synonyms
- zgc:110642 antibody, LNCR2 antibody, Acra-5 antibody, Acra5 antibody, cholinergic receptor, nicotinic, alpha 5 antibody, cholinergic receptor nicotinic alpha 5 subunit antibody, cholinergic receptor nicotinic alpha 5 subunit L homeolog antibody, cholinergic receptor, nicotinic, alpha polypeptide 5 antibody, chrna5 antibody, CHRNA5 antibody, chrna5.L antibody, Chrna5 antibody
- Background
- CHRNA5, cholinergic receptor, nicotinic, alpha 5, LNCR2, Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5, neuronal acetylcholine receptor subunit alpha-5, neuronal nicotinic acetylcholine receptor, alpha5 subunit
- Molecular Weight
- 53.1kDa according to NP_000736.2
- Gene ID
- 1138
- NCBI Accession
- NP_000736
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