IL7R antibody (Internal Region)

Catalog No. ABIN768570

This anti-IL7R antibody is a Goat Polyclonal antibody detecting IL7R in ELISA. Suitable for Mouse.

Quick Overview for IL7R antibody (Internal Region) (ABIN768570)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IL7R antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-IL7R Antibody

Binding Specificity: Internal Region

Purpose: CD127 / IL7R (mouse)

Sequence: DDHSFWCHSQ LE

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence DDHSFWCHSQLE, from the internal region (near N Terminus) of the protein sequence according to NP_032398.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Preliminary testing showed a band at approx 50 kDa in NIH3T3 cell lysate, and 55 kDa in Mouse Duodenum lysate after 1-2 μg/mL antibody staining (calculated MW of 51.6 kDa according to Mouse NP_032398.3). Primary incubation 1 hour at room tempe

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: IL7R

Background: IL7R, interleukin 7 receptor, CD127, IL-7R-alpha, IL7RA, ILRA, CD127 antigen, IL-7 receptor subunit alpha, IL-7R subunit alpha, IL-7RA, interleukin 7 receptor alpha chain, interleukin 7 receptor isoform H5-6, interleukin-7 receptor subunit alpha, MGC10755

Molecular Weight: 51.6kDa according to NP_032398.3

Gene ID: 16197, 294797

NCBI Accession: NP_032398

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size