BPIFA2 antibody (AA 146-160)

Catalog No. ABIN768610

This Goat Polyclonal antibody specifically detects BPIFA2 in WB, ELISA and ICC. It exhibits reactivity toward Mouseand has been mentioned in 1 publication.

Quick Overview for BPIFA2 antibody (AA 146-160) (ABIN768610)

Target: BPIFA2 (BPI Fold Containing Family A, Member 2 (BPIFA2))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This BPIFA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-BPIFA2 Antibody

Binding Specificity: AA 146-160

Purpose: parotid secretory protein (mouse aa146-160)

Sequence: KTNAQTGLPE VTIGK

Cross-Reactivity: Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KTNAQTGLPEVTIGK, from the internal region of the protein sequence according to NP_032979.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 25 kDa band observed in Mouse mandibular glands from E17 onwards (calculated MW of 24.8 kDa according to NP_032979.1). Recommended concentration: 1-3 μg/mL. Data provided by Everest Grant winner Melinda Larsen, State University of New Y

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunocytochemistry: A subset of murine submandibular gland cells (E18) show specific staining in secretory granules. Recommended concentration 4ug/ml. Data provided by Everest Grant winner Melinda Larsen, State University of New York, Al

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-BPIFA2 antibody (ABIN768610)

Tanaka, Ogawa, Hojo, Kawashima, Mabuchi, Hata, Nakamura, Yasuhara, Takamatsu, Irié, Fukada, Sakai, Inoue, Nishimura, Ohara, Saito, Ohba, Tsuji, Mishima: "Generation of orthotopically functional salivary gland from embryonic stem cells." in: Nature communications, Vol. 9, Issue 1, pp. 4216, (2019) (PubMed).

Target Details for BPIFA2

Target: BPIFA2 (BPI Fold Containing Family A, Member 2 (BPIFA2))

Alternative Name: Bpifa2

Background: Psp, parotid secretory protein, Bpifa2e, OTTMUSP00000018314, OTTMUSP00000036473

Gene ID: 19194

NCBI Accession: NP_032979