BTBD7 antibody (N-Term)

Catalog No. ABIN768612

This anti-BTBD7 antibody is a Goat Polyclonal antibody detecting BTBD7 in WB and ELISA. Suitable for Mouse. This Primary Antibody has been cited in 1 publication.

Quick Overview for BTBD7 antibody (N-Term) (ABIN768612)

Target: BTBD7 (BTB (POZ) Domain Containing 7 (BTBD7))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This BTBD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-BTBD7 Antibody

Binding Specificity: N-Term

Purpose: BTBD7

Sequence: ESKLYSLDHG HEKPQ

Specificity: This antibody is expected to recognize both reported isoforms (NP_001002860.2, NP_060637.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ESKLYSLDHGHEKPQ-C, from the N Terminus of the protein sequence according to NP_001002860.2, NP_060637.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected MDCK transiently expressing the fusion protein Myc-Btbd7-GFP a band of approx. 190 kDa is observed. This band is also observed by Myc-antibody and it is not observed in the MDCK expressing GFP, or when the fusion gene was supp

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-BTBD7 antibody (ABIN768612)

Grisard, Coan, Cesaratto, Rigo, Zandonà, Paulitti, Andreuzzi, Rampioni Vinciguerra, Poletto, Del Ben, Brisotto, Biscontin, Turetta, Dassi, Mirnezami, Canzonieri, Vecchione, Baldassarre, Mongiat et al.: "Sleeping beauty genetic screen identifies miR-23b::BTBD7 gene interaction as crucial for colorectal cancer metastasis. ..." in: EBioMedicine, Vol. 46, pp. 79-93, (2020) (PubMed).

Target Details for BTBD7

Target: BTBD7 (BTB (POZ) Domain Containing 7 (BTBD7))

Alternative Name: BTBD7

Background: BTBD7, BTB (POZ) domain containing 7, DKFZp686N0544, FUP1, MGC48310, BTB/POZ domain-containing protein 7

Molecular Weight: 36kDa

Gene ID: 55727, 238386, 362772

NCBI Accession: NP_001002860, NP_060637