ZIC2 antibody (C-Term)

Catalog No. ABIN768616

This Goat Polyclonal antibody specifically detects ZIC2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ZIC2 antibody (C-Term) (ABIN768616)

Target: ZIC2

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ZIC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-ZIC2 Antibody

Binding Specificity: C-Term

Purpose: ZIC2

Sequence: HSGLSSNFNE WY

Specificity: This antibody is expected to NOT cross-react with other ZIC proteins.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-HSGLSSNFNEWY, from the C Terminus of the protein sequence according to NP_009060.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65 kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0 kDa accordin

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ZIC2

Target: ZIC2

Alternative Name: ZIC2

Background: ZIC2, Zic family member 2 (odd-paired homolog, Drosophila), HPE5, Zic family member 2 (odd-paired Drosophila homolog), Zinc finger protein of the cerebellum 2, zinc finger protein ZIC 2

Molecular Weight: 75kDa

Gene ID: 7546, 22772, 361096

NCBI Accession: NP_009060

Pathways: Tube Formation