CMA1 antibody (Internal Region)

Catalog No. ABIN768626

The Goat Polyclonal anti-CMA1 antibody has been validated for WB, ELISA and IHC. It is suitable to detect CMA1 in samples from Human.

Quick Overview for CMA1 antibody (Internal Region) (ABIN768626)

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CMA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CMA1 Antibody

Binding Specificity: Internal Region

Purpose: CMA1

Sequence: QFRHPKYNTS TLHHD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QFRHPKYNTSTLHHD, from the internal region of the protein sequence according to NP_001827.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Testis and Tonsil (Mast cells). Recommended concentration: 1.5 μg/mL.

Western Blot: In transfected HEK293 transiently expressing full-length Human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35 kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag an

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CMA1

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Alternative Name: CMA1

Background: CMA1, chymase 1, mast cell, CYH, MCT1, MGC119890, MGC119891, chymase 1 preproprotein transcript E, chymase 1 preproprotein transcript I, chymase, heart, chymase, mast cell, mast cell protease I

Molecular Weight: 35kDa

Gene ID: 1215

NCBI Accession: NP_001827

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis