MKRN1 antibody (AA 105-118)

Catalog No. ABIN768634

This Goat Polyclonal antibody specifically detects MKRN1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for MKRN1 antibody (AA 105-118) (ABIN768634)

Target: MKRN1 (Makorin Ring Finger Protein 1 (MKRN1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This MKRN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-MKRN1 Antibody

Binding Specificity: AA 105-118

Purpose: MKRN1 (aa105-118)

Sequence: RYEHSKPLKQ EEAT

Specificity: This antibody is expected to recognize both reported isoforms (NP_038474.2, NP_001138597.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RYEHSKPLKQEEAT, from the internet regoin of the protein sequence according to NP_038474.2, NP_001138597.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 50 kDa band observed in fetal Mouse Brain lysates and 35 kDa plus fainter 50 kDa bands in adult Mouse Brain lysates (calculated MW of 52.9 kDa according to NP_061280.2). The observed 35 kDa band corresponds to the calculated MW of human i

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email sales@everestbiotech.com to request datasheet, pricing and delivery information.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MKRN1

Target: MKRN1 (Makorin Ring Finger Protein 1 (MKRN1))

Alternative Name: MKRN1

Background: MKRN1, makorin ring finger protein 1, FLJ21334, RNF61, E3 ubiquitin-protein ligase makorin-1, OTTHUMP00000212121, OTTHUMP00000212124, RING finger protein 61

Molecular Weight: 60kDa

Gene ID: 23608, 54484

NCBI Accession: NP_038474, NP_001138597

Pathways: Chromatin Binding