DBF4 antibody (Internal Region)

Catalog No. ABIN768659

The Goat Polyclonal anti-DBF4 antibody has been validated for WB and ELISA. It is suitable to detect DBF4 in samples from Mouse and Rat.

Quick Overview for DBF4 antibody (Internal Region) (ABIN768659)

Target: DBF4 (DBF4 Homolog (DBF4))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This DBF4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-DBF4 Antibody

Binding Specificity: Internal Region

Purpose: Dbf4 (mouse)

Sequence: DKKRTEYLPA HEDRT

Specificity: This antibody is expected to recognize both reported isoforms (NP_038754.1, NP_001177646.1).

Cross-Reactivity: Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DKKRTEYLPAHEDRT, from the internal region of the protein sequence according to NP_038754.1, NP_001177646.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Mouse and Rat Testis and Thymus lysates (calculated MW of 74.2 kDa according to NP_038754.1). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DBF4

Target: DBF4 (DBF4 Homolog (DBF4))

Alternative Name: Dbf4

Background: Dbf4, DBF4 homolog (S. cerevisiae), AA545217, Ask, activator of S phase kinase, protein DBF4 homolog A

Molecular Weight: 74.2kDa according to NP_038754.1

Gene ID: 27214, 312046

NCBI Accession: NP_038754, NP_001177646

Pathways: Mitotic G1-G1/S Phases, DNA Replication