Glutamate Receptor 1 antibody (AA 264-277)

Catalog No. ABIN768662

The Goat Polyclonal anti-Glutamate Receptor 1 antibody has been validated for WB and ELISA. It is suitable to detect Glutamate Receptor 1 in samples from Human.

Quick Overview for Glutamate Receptor 1 antibody (AA 264-277) (ABIN768662)

Target: Glutamate Receptor 1 (GLUR1)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Glutamate Receptor 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-Glutamate Receptor 1 Antibody

Binding Specificity: AA 264-277

Purpose: AMPA1 / GLUR1 (aa264-277)

Sequence: KIMQQWKNSD ARD

Specificity: This antibody is expected to recognize both reported isoforms (NP_000818.2, NP_001107655.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KIMQQWKNSDARD, from the internal region of the protein sequence according to NP_000818.2, NP_001107655.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 100 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 102 kDa according to NP_000818.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Glutamate Receptor 1

Target: Glutamate Receptor 1 (GLUR1)

Alternative Name: GRIA1

Background: GRIA1, glutamate receptor, ionotropic, AMPA 1, GLUH1, GLUR1, GLURA, GluA1, HBGR1, MGC133252, AMPA 1, AMPA-selective glutamate receptor 1, gluR-1, gluR-A, gluR-K1, glutamate receptor 1

Molecular Weight: 102kDa according to NP_000818.2

Gene ID: 2890, 14799, 50592

NCBI Accession: NP_000818, NP_001107655

Pathways: PI3K-Akt Signaling