Keratin Basic antibody

Catalog No. ABIN768698

Product Details

Target: Keratin Basic

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: IHC-plus™

Specificity: This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is broadly reactive with almost all epithelial and there neoplasms. This antibody together with AE-1 has been used for studying the immuno-localization of epidermal keratins, tissue distribution and epithelia development. AE-1 and AE-3 have also been used extensively, individually or mixed, for distinguishing carcinoma from non-carcinoma. The antibody is cross-reactive with keratins of rabbit, mouse, rat, bovine and chicken. Within immunoblots AE-1 detects distinct MW's of 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip's lower then 6.0. These MW's are believed to correspond with respectively keratin 8, 7, 6, 5, 4, 3 and 1 & 2.

Purification: Purified IgG

Immunogen: Human epidermal cells.

Type of Immunogen: Cells

Clone: AE3

Isotype: IgG1

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (1:100), WB (1:100)

Usage: In unfixed frozen sections and formalin-fixed paraffin embedded tissues. Also applicable in Western blotting. For use on formalin-fixed paraffin embedded tissues a dilution of approximately 1:150 to 1:200 is appropriate if section are incubated for 30 min. at 37 °C. Possibly, higher dilutions can be used if sections are incubated at 4 °C overnight. For use in Western blotting a dilution of 1:100 is advised.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS containing 0.1 % Sodium azide and 1 % BSA. Sourced in Ascites.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: Keratin Basic

Alternative Name: Basic Cytokeratin (Keratin Basic Products)