CD90 antibody (PE)

Catalog No. ABIN780368

Product Details anti-CD90 Antibody (PE)

Target: CD90 (THY1)

Reactivity: Human, Pig, Horse, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody 5E10 recognizes CD90/Thy-1, a GPI-anchored cell surface glycoprotein expressed predominantly on thymocytes, hematopoietic stem cells and neurons.

No Cross-Reactivity: Dog

Cross-Reactivity (Details): Human, Non-Human Primates, Porcine, Equine (Horse)

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: HEL erythroleukemia cells

Clone: 5E10

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD90 antibody (PE) (ABIN780368)

DePompeo, Giassetti, Elnaggar, Oatley, Davis, Fransson: "Isolation of canine adipose-derived mesenchymal stem cells from falciform tissue obtained via laparoscopic morcellation: A pilot study." in: Veterinary surgery : VS, (2019) (PubMed).

Hoppstädter, Diesel, Zarbock, Breinig, Monz, Koch, Meyerhans, Gortner, Lehr, Huwer, Kiemer: "Differential cell reaction upon Toll-like receptor 4 and 9 activation in human alveolar and lung interstitial macrophages." in: Respiratory research, Vol. 11, pp. 124, (2010) (PubMed).

Kroeze, Jurgens, Doulabi, van Milligen, Scheper, Gibbs: "Chemokine-mediated migration of skin-derived stem cells: predominant role for CCL5/RANTES." in: The Journal of investigative dermatology, Vol. 129, Issue 6, pp. 1569-81, (2009) (PubMed).

Carlsten, Björkström, Norell, Bryceson, van Hall, Baumann, Hanson, Schedvins, Kiessling, Ljunggren, Malmberg: "DNAX accessory molecule-1 mediated recognition of freshly isolated ovarian carcinoma by resting natural killer cells." in: Cancer research, Vol. 67, Issue 3, pp. 1317-25, (2007) (PubMed).

Seiffert, Brossart, Cant, Cella, Colonna, Brugger, Kanz, Ullrich, Bühring: "Signal-regulatory protein alpha (SIRPalpha) but not SIRPbeta is involved in T-cell activation, binds to CD47 with high affinity, and is expressed on immature CD34(+)CD38(-) hematopoietic cells." in: Blood, Vol. 97, Issue 9, pp. 2741-9, (2001) (PubMed).

St Louis, Woodcock, Franzoso, Blair, Carlson, Murillo, Wells, Williams, Smoot, Kaushal, Grimes, Harlan, Chute, June, Siebenlist, Lee, Fransozo: "Evidence for distinct intracellular signaling pathways in CD34+ progenitor to dendritic cell differentiation from a human cell line model." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 6, pp. 3237-48, (1999) (PubMed).

Ito, Inaba, Inaba, Toki, Sogo, Iguchi, Adachi, Yamaguchi, Amakawa, Valladeau, Saeland, Fukuhara, Ikehara: "A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 3, pp. 1409-19, (1999) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: Thy-1 cell surface antigen,CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.,Thy-1

Gene ID: 7070

UniProt: P04216

Pathways: Cell-Cell Junction Organization