CD20 antibody

Catalog No. ABIN781718

The Mouse Monoclonal anti-CD20 antibody is suitable to detect CD20 in samples from Human. It has been validated for FACS and IHC (fro).

Quick Overview for CD20 antibody (ABIN781718)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CAT13-6E12

Product Details anti-CD20 Antibody

Specificity: This antibody detects CD20.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Affinity chromatography

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute by adding 0.5 mL distilled water.

Buffer: Stock solution contains 0.2 mg/mL IgG, phosphate buffered saline pH 7.2 (PBS), 5 mg/mL bovine serum albumin (BSA), and 0.09 % sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store (in aliquots) at -20 °C.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Background: CD20 belongs to the MS4A family (Membrane-spanning 4-domains subfamily A). Its expression is restricted to normal and neoplastic B cells. It is expressed by pre-B cells in the bone marrow, and expression persists during B cell maturation but is lost upon terminal differentiation into plasma cells. CD20 is not glycosylated. CD20 is not phosphorylated in resting B cells, but becomes heavily phosphorylated after mitogen stimulation. Three cell surface isoforms 33, 35 and 37kD, result from differential phosphorylation of a single core 33kD protein within the cytoplasmic domain. Multiple proteins are coimmunoprecipitated with CD20, suggesting that it may be a component of a multimeric surface receptor complex. Monoclonal antibodies against CD20 are used therapeutically, such as Rituximab (Mabthera) or Britumomab (Zevalin).Synonyms: B-cell marker, B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leu-16, MS4A1, Membrane-spanning 4-domains subfamily A member 1

Gene ID: 931

NCBI Accession: NP_068769

UniProt: P11836