Nitrotyrosine antibody

Catalog No. ABIN781807

This Mouse Monoclonal antibody specifically detects Nitrotyrosine in WB, IHC (fro) and IHC (p).

Quick Overview for Nitrotyrosine antibody (ABIN781807)

Target: Nitrotyrosine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EM-30

Product Details anti-Nitrotyrosine Antibody

Purification: Affinity Chromatography

Immunogen: NO2-Tyr-CH2-Thyroglobulin

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical

Background: Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.Synonyms: NO-Tyrosine, Nitro-Tyrosine