SMC1A antibody

Catalog No. ABIN781888

This Mouse Monoclonal antibody specifically detects SMC1A in WB, IHC (p) and EIA. It exhibits reactivity toward Human.

Quick Overview for SMC1A antibody (ABIN781888)

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMC1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 1B9

Product Details anti-SMC1A Antibody

Specificity: This antibody reacts to SMC1A / SMC1.

Purification: Protein A chromatography

Immunogen: SMC1A antibody was raised against recombinant protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0,32 mg/mL

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SMC1A

Target: SMC1A (Structural Maintenance of Chromosomes 1A (SMC1A))

Alternative Name: SMC1A / SMC1

Background: Structural maintenance of chromosome proteins (SMC) are involved in chromosome cohesion during cell cycle and in DNA repair as part of the of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint The form of SMC1A phosphorylated at Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function.Synonyms: DXS423E, SB1.8, SMC protein 1A, SMC-1-alpha, SMC-1A, SMC1L1, Structural maintenance of chromosomes protein 1A

Gene ID: 8243

NCBI Accession: NP_006297

UniProt: Q14683

Pathways: Stem Cell Maintenance