ASAH1 antibody (AA 25-125)

Catalog No. ABIN782337

Product Details anti-ASAH1 Antibody

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Binding Specificity: AA 25-125

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ASAH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: Recognizes Acid ceramidase ASAH1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein A Chromatography

Immunogen: ASAH1 antibody was raised against ASAH1 (NP_808592, 25 a.a. ~ 125 a.a) partial recombinant protein with GST tag.

Clone: 1A7

Isotype: IgG3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20° or -80 °C.

Target Details for ASAH1

Target: ASAH1 (N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1))

Alternative Name: Acid Ceramidase (ASAH1 Products)

Background: Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH1 is an acid ceramidase and key regulator of ceramide metabolism. Mutations in this gene results in Farber Lipogranulomatosis, a fatal human genetic disorder that results in the painful swelling of the joints and tendons and pulminary insufficiency, while a complete knockout of its expression is lethal in mice. Recent studies have shown elevated levels of ASAH1 in Alzheimer's disease (AD) patients correlating with a reduction in sphingomyelin and elevation of ceramide. Pretreatment of cultured neurons with recombinant AHAH1 prevented the cells from undergoing A-beta (Ab)-induced apoptosis. Multiple isoforms of this protein are known to exist.Synonyms: ASAH, ASAH1, Acylsphingosine deacylase, N-acylsphingosine amidohydrolase, PHP32, Putative 32 kDa heart protein

Gene ID: 427

NCBI Accession: NP_001120977

UniProt: Q13510