BCL9L antibody (N-Term)

Catalog No. ABIN783409

This Rabbit Polyclonal antibody specifically detects BCL9L in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BCL9L antibody (N-Term) (ABIN783409)

Target: BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL9L antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-BCL9L Antibody

Binding Specificity: N-Term

Specificity: This antibody detects Bcl-9-like protein at N-term. It is predicted to not cross-react with other Bcl family members. At least four isoforms of Bcl9L are known to exist, this antibody will detect all four.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography purified via peptide column

Immunogen: 20 amino acid peptide near the amino terminus of human Bcl9L

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for BCL9L

Target: BCL9L (B-Cell CLL/lymphoma 9-Like (BCL9L))

Alternative Name: Bcl-9-Like Protein

Background: Bcl9L, a homolog of Bcl9, was initially identified through a bioinformatics screening. It is expressed in fetal brain, adult lung, eye and prostate, in addition to several types of tumors including pancreatic and prostate cancers. Bcl9L has been shown to interact with b-catenin, a target of the Wnt signaling pathway, and is required for enhanced ?-catenin-T-cell factor (TCF)-mediated transcription in colorectal tumor cells, possibly by translocating b-catenin to the nucleus. Other studies have indicated that Bcl9L expression correlates with high nuclear grade cancer phenotype and the expression of ErbB2/HER-2 in breast cancers, suggesting that activity may occur in other types of cancer. Bcl9L has also been shown to be critical for Wnt-mediate regulation of stem cell traits in colon epithelium and adenocarcinomas which are associated with tumor invasion, metastasis, and resistance to therapy.Synonyms: B-cell CLL/lymphoma 9-like protein, B-cell lymphoma 9-like protein, BCL9-like protein, BCL9L, DLNB11

Gene ID: 283149

NCBI Accession: NP_872363

UniProt: Q86UU0

Pathways: Stem Cell Maintenance