Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Geminin Coiled-Coil Domain Containing (GMNC) (Center) antibody Primary Antibody

GMNC Reactivity: Human EIA, WB Host: Rabbit Polyclonal
Catalog No. ABIN783498
$511.50
Plus shipping costs $45.00
0.1 mg
local_shipping Shipping to: United States
Delivery in 6 to 8 Business Days
  • Target
    Binding Specificity
    Center
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Enzyme Immunoassay (EIA), Western Blotting (WB)
    Specificity
    This antibody detects GEMC1 at Center.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, mouse
    Purification
    Affinity chromatography purified via peptide column
    Immunogen
    19 amino acid peptide near the center of human GEMC1
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Buffer
    PBS containing 0.02 % sodium azi
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.
  • Target
    Alternative Name
    GEMC1 (GMNC Antibody Abstract)
    Background
    The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.Synonyms: Geminin coiled-coil domain-containing protein 1
    Gene ID
    647309
    NCBI Accession
    NP_001140158
    UniProt
    A6NCL1
You are here: