GEMC1 antibody (Center)

Catalog No. ABIN783498

The Rabbit Polyclonal anti-GEMC1 antibody (ABIN783498) specifically detects GEMC1 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for GEMC1 antibody (Center) (ABIN783498)

Target: GEMC1 (GMNC) (Geminin Coiled-Coil Domain Containing (GMNC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GEMC1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GEMC1 Antibody

Binding Specificity: Center

Specificity: This antibody detects GEMC1 at Center.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse

Purification: Affinity chromatography purified via peptide column

Immunogen: 19 amino acid peptide near the center of human GEMC1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azi

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer.

Target Details for GEMC1

Target: GEMC1 (GMNC) (Geminin Coiled-Coil Domain Containing (GMNC))

Alternative Name: GEMC1

Background: The Geminin coiled-coil domain-containing protein 1 (GEMC1) is a highly conserved protein that is required for chromosomal replication. GEMC1 contains a region similar to the coiled-coil domain of geminin, an essential vertebrate replication protein in which the coiled-coil domain is required for the function. GEMC1 binds to the checkpoint and replication factor TopBP1, allowing the binding of GEMC1 to chromatin during pre-replication complex formation. GEMC1 also directly interacts with replication factors Cdc45 and Cdk2-CyclinE and stimulates the initiation of DNA replication. Depletion of GEMC1 by siRNA in embryonic and somatic vertebrate cells prevents DNA replication due to impairment of Cdc45 loading onto chromatin, suggesting GEMC1 promotes initiation of DNA replication by mediating TopBP1 and Cdk2 dependent recruitment of Cdc45 onto replication origins.Synonyms: Geminin coiled-coil domain-containing protein 1

Gene ID: 647309

NCBI Accession: NP_001140158

UniProt: A6NCL1