RNF8 antibody (C-Term)

Catalog No. ABIN783604

This Rabbit Polyclonal antibody specifically detects RNF8 in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RNF8 antibody (C-Term) (ABIN783604)

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-RNF8 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Affinity chromatography purified via peptide column

Immunogen: 14 amino acid peptide near the carboxy terminus of human RNF8

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for RNF8

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Alternative Name: RNF8

Background: RNF8 was identified as a ubiquitin ligase (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A histone ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.Synonyms: E3 ubiquitin-protein ligase RNF8, RING finger protein 8

Gene ID: 9025

NCBI Accession: NP_003949

UniProt: O76064

Pathways: Production of Molecular Mediator of Immune Response