HGS antibody (Internal Region)

Catalog No. ABIN784218

The Rabbit Polyclonal anti-HGS antibody (ABIN784218) specifically detects HGS in WB, IF, IHC (p) and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HGS antibody (Internal Region) (ABIN784218)

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HGS antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-HGS Antibody

Binding Specificity: Internal Region

Specificity: This antibody detects endogenous levels of total HGS protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated Remarks: Antigen Modification: Internal

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM Sodium Chloride, 0.02 % Sodium Azide and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HGS

Target: HGS (Hepatocyte Growth Factor-Regulated tyrosine Kinase Substrate (HGS))

Alternative Name: HGS / HRS

Background: HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. It could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. HGS may concentrate ubiquitinated receptors within clathrin-coated regions. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM. This complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex.Synonyms: Hepatocyte growth factor-regulated tyrosine kinase substrate, Protein pp110

Gene ID: 9146

NCBI Accession: NP_004703

Pathways: EGFR Signaling Pathway, CXCR4-mediated Signaling Events, Synaptic Vesicle Exocytosis, EGFR Downregulation