LPO antibody (Biotin)

Catalog No. ABIN784497

This anti-LPO antibody is a Rabbit Polyclonal antibody detecting LPO in WB, IP, EIA, DB, ID, IF and RIA. Suitable for Cow.

Quick Overview for LPO antibody (Biotin) (ABIN784497)

Target: LPO (Lactoperoxidase (LPO))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LPO antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunofluorescence (IF), Radioimmunoassay (RIA)

Product Details anti-LPO Antibody (Biotin)

Specificity: The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of bovine serum. Interspecies cross-reactivity is a normal feature of antibodies, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, but reactivity with lactoperoxidase from goat and sheep may be expected.

Purification: DEAE-column Chromatography

Immunogen: Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80,000. It is isolated from pooled bovine milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore by adding 1 mL sterile distilled water

Concentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.

Target Details for LPO

Target: LPO (Lactoperoxidase (LPO))

Alternative Name: Lactoperoxidase

Background: Lactoperoxidase is a natural antibacterial agent in bovine milk, a medium otherwise rich in its ability to support bacterial growth. Milk lactoperoxidase levels vary among species, ranging from little or none in humans to very high concentrations in guinea pigs. Interestingly, lactoperoxidase from milk is identical to the lacrimal and salivary forms of the enzyme. In addition, the enzyme catalyzes the hydrogen peroxide oxidation of iodine and can be employed in labelling proteins with radioiodine.Synonyms: EC=1.11.1.7, LPO, Lactoperoxidase, SAPX, SPO, Salivary peroxidase

Gene ID: 280844

NCBI Accession: NP_776358