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LPO antibody (Biotin)

LPO Reactivity: Cow WB, IP, EIA, DB, ID, IF, RIA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN784497
  • Target See all LPO Antibodies
    LPO (Lactoperoxidase (LPO))
    Reactivity
    • 12
    • 8
    • 7
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Cow
    Host
    • 16
    • 4
    • 2
    Rabbit
    Clonality
    • 20
    • 2
    Polyclonal
    Conjugate
    • 13
    • 7
    • 2
    This LPO antibody is conjugated to Biotin
    Application
    • 17
    • 12
    • 9
    • 8
    • 7
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Dot Blot (DB), Immunodiffusion (ID), Immunofluorescence (IF), Radioimmunoassay (RIA)
    Specificity
    The reactivity of the antiserum is restricted to lactoperoxidase as tested in immunoelectrophoresis and radial immunodiffusion. Using various antiserum concentrations against fresh bovine milk a single precipitin line is obtained. No precipitation reaction is obtained with normal bovine serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of bovine serum. Interspecies cross-reactivity is a normal feature of antibodies, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, but reactivity with lactoperoxidase from goat and sheep may be expected.
    Purification
    DEAE-column Chromatography
    Immunogen
    Lactoperoxidase (EC 1.11.1.7) is one of the major enzymes in bovine milk. The enzyme has a molecular weight of 80,000. It is isolated from pooled bovine milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Reconstitution
    Restore by adding 1 mL sterile distilled water
    Concentration
    10 mg/mL
    Buffer
    PBS, pH 7.2 without preservatives and foreign proteins.
    Preservative
    Without preservative
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -20 °C for longer.
  • Target
    LPO (Lactoperoxidase (LPO))
    Alternative Name
    Lactoperoxidase (LPO Products)
    Synonyms
    LPO antibody, SPO antibody, 5830499B15Rik antibody, lactoperoxidase antibody, LPO antibody, Lpo antibody
    Background
    Lactoperoxidase is a natural antibacterial agent in bovine milk, a medium otherwise rich in its ability to support bacterial growth. Milk lactoperoxidase levels vary among species, ranging from little or none in humans to very high concentrations in guinea pigs. Interestingly, lactoperoxidase from milk is identical to the lacrimal and salivary forms of the enzyme. In addition, the enzyme catalyzes the hydrogen peroxide oxidation of iodine and can be employed in labelling proteins with radioiodine.Synonyms: EC=1.11.1.7, LPO, Lactoperoxidase, SAPX, SPO, Salivary peroxidase
    Gene ID
    280844
    NCBI Accession
    NP_776358
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