p300 antibody (pSer1834) (Cy5.5)

Catalog No. ABIN800618

The Rabbit Polyclonal anti-p300 antibody has been validated for WB, IF (cc) and IF (p). It is suitable to detect p300 in samples from Human, Mouse and Zebrafish (Danio rerio).

Quick Overview for p300 antibody (pSer1834) (Cy5.5) (ABIN800618)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-p300 Antibody (Cy5.5)

Binding Specificity: pSer1834

Cross-Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse,Chicken,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Ep300 around the phosphorylation site of Ser1834

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: Ep300

Background:

Synonyms: CREBBP/EP300 inhibitory protein 1, Cyclic AMP responsive enhancer binding protein, E1A associated protein p300, E1A binding protein p300, EC 2.3.1.48, EP300, EP300: E1A binding protein p300, RB and P300 binding protein EID 1, Histone acetyltransferase p300, p300 HAT, Retinoblastoma protein associated protein.

Background: KAT3B/p300 is a transcriptional adapter protein (300 kDa)which is characterized by three cysteine and histidine rich regions and its C-terminus specifically binds the adenovirus E1A protein. KAT3B and associated proteins are components of TATA-binding protein (TBP)complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265 kDa nuclear protein designated KAT3A/CBP(for CREB-binding protein). KAT3B and KAT3A are homologous to each other.

Gene ID: 2033

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size