HSF1 antibody (pSer303)

Catalog No. ABIN800958

This anti-HSF1 antibody is a Rabbit Polyclonal antibody detecting HSF1 in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). Suitable for Human.

Quick Overview for HSF1 antibody (pSer303) (ABIN800958)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-HSF1 Antibody

Binding Specificity: pSer303

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human HSF1 around the phosphorylation site of Ser303

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background:

Synonyms: HSF1Ser303, Heat shock factor 1, Heat shock factor protein 1, Heat shock transcription factor 1, HSF 1, hsf1, HSTF 1, HSTF1.

Background: Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38 % sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells.HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.

Gene ID: 3297