MAP2K5 antibody (pSer311, pThr315) (Biotin)

Catalog No. ABIN801950

The Rabbit Polyclonal anti-MAP2K5 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect MAP2K5 in samples from Mouse.

Quick Overview for MAP2K5 antibody (pSer311, pThr315) (Biotin) (ABIN801950)

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP2K5 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MAP2K5 Antibody (Biotin)

Binding Specificity: pSer311, pThr315

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MEK5 around the phosphorylation site of Ser311+Thr315

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for MAP2K5

Target: MAP2K5 (Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5))

Alternative Name: MEK5+

Background:

Synonyms: MAP2K5 phospho S311/T315, Dual specicity mitogen activated protein kinase kinase 5, MAP kinase kinase 5, MAP kinase kinase MEK5b, MAP2K5, MAPK/ERK kinase 5, MAPKK5, PRKMK5, EC 2.7.12.2.

Background: MEK5 is a dual specificity protein kinase belonging to thr Ser/Thr protein kinase family, (MAP kinase kinase family). It is activated by phosphorylation on Ser/Thr by MAP kinase kinases and interacts specifically with ERK5, and not with another MAP kinase like P38. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). It is not phosphorylated by RAFA, RAFB or RAFC and it may interact with GTPases such as CDC42. The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. MEK5 is expressed in many adult tissues and is most abundant in heart and skeletal muscle.

Gene ID: 5607

UniProt: Q13163

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway