MCAM antibody (pTyr641)

Catalog No. ABIN802563

This anti-MCAM antibody is a Rabbit Polyclonal antibody detecting MCAM in WB and ELISA. Suitable for Human and Rat.

Quick Overview for MCAM antibody (pTyr641) (ABIN802563)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MCAM Antibody

Binding Specificity: pTyr641

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MCAM around the phosphorylation site of Tyr641

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: MCAM

Background:

Synonyms: Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen, MCAM, MUC18

Background: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Gene ID: 4162