Rhodopsin antibody

Catalog No. ABIN863085

The Mouse Monoclonal anti-Rhodopsin antibody has been validated for WB, IHC, ELISA, IP, ICC and IF. It is suitable to detect Rhodopsin in samples from Cow. There are 2+ publications available.

Quick Overview for Rhodopsin antibody (ABIN863085)

Target: Rhodopsin (RHO)

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Rhodopsin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: 1D4

Product Details anti-Rhodopsin Antibody

Specificity: Detects ~40 kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).

Cross-Reactivity: Vertebrate

Purification: Protein G Purified

Immunogen: Bovine Rhodopsin

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN863084 was sufficient for detection of rhodopsin in 10 μg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-Rhodopsin antibody (ABIN863085)

Makowka, Bruegmann, Dusend, Malan, Beiert, Hesse, Fleischmann, Sasse: "Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision." in: Nature communications, Vol. 10, Issue 1, pp. 1281, (2019) (PubMed).

Cheng, Djajadi, Molday: "Cell-specific markers for the identification of retinal cells by immunofluorescence microscopy." in: Methods in molecular biology (Clifton, N.J.), Vol. 935, pp. 185-99, (2012) (PubMed).

Target Details for Rhodopsin

Target: Rhodopsin (RHO)

Alternative Name: Rhodopsin

Target Type: Chemical

Background: Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Gene ID: 509933

NCBI Accession: NP_001014890

UniProt: P02699

Pathways: WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction