APOB antibody

Catalog No. ABIN865414

This Mouse Monoclonal antibody specifically detects APOB in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for APOB antibody (ABIN865414)

Target: APOB (Apolipoprotein B (APOB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APOB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 6G6

Product Details anti-APOB Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human ApoB expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FC: 1/200 - 1/400 ELISA: Propose dilution 1/10000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100g/100l

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for APOB

Target: APOB (Apolipoprotein B (APOB))

Alternative Name: ApoB

Background: This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. Synonyms: FLDB, LDLCQ4

Molecular Weight: 516kDa

Gene ID: 338

Pathways: Lipid Metabolism