CRTC2 antibody

Catalog No. ABIN865510

Product Details anti-CRTC2 Antibody

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRTC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CRTC2 expressed in E. Coli.

Clone: 5B10

Isotype: IgG1

Application Details

Application Notes: WB:1/500 - 1/2000,IHC:1/200 - 1/1000,IF:1/200-1/1000,FC:1/200-1/400, ELISA: Propose dilution 1/10000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100g/100l

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CRTC2

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Alternative Name: CRTC2 (CRTC2 Products)

Background: Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic -cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes -cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling . The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways . In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Synonyms: TORC2, TORC-2, CRTC2

Molecular Weight: 80kDa

Gene ID: 200186

Pathways: AMPK Signaling, Carbohydrate Homeostasis