CRTC2 antibody

Catalog No. ABIN865510

This Mouse Monoclonal antibody specifically detects CRTC2 in WB, ELISA, IHC, IF and FACS. It exhibits reactivity toward Human and Monkey.

Quick Overview for CRTC2 antibody (ABIN865510)

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRTC2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 5B10

Product Details anti-CRTC2 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CRTC2 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: WB:1/500 - 1/2000,IHC:1/200 - 1/1000,IF:1/200-1/1000,FC:1/200-1/400, ELISA: Propose dilution 1/10000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100g/100l

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CRTC2

Target: CRTC2 (CREB Regulated Transcription Coactivator 2 (CRTC2))

Alternative Name: CRTC2

Background: Glucose homeostasis is regulated by hormones and cellular energy status. Elevations of blood glucose during feeding stimulate insulin release from pancreatic -cells through a glucose sensing pathway. Feeding also stimulates release of gut hormones such as glucagon-like peptide-1 (GLP-1), which further induces insulin release, inhibits glucagon release and promotes -cell viability. CREB-dependent transcription likely plays a role in both glucose sensing and GLP-1 signaling . The protein Torc2 (transducer of regulated CREB activity 2) functions as a CREB co-activator and is implicated in mediating the effects of these two pathways . In quiescent cells, Torc2 is phosphorylated at Ser171 and becomes sequestered in the cytoplasm via an interaction with 14-3-3 proteins. Glucose and gut hormones lead to the dephosphorylation of Torc2 and its dissociation from 14-3-3 proteins. Dephosphorylated Torc2 enters the nucleus to promote CREB-dependent transcription. Synonyms: TORC2, TORC-2, CRTC2

Molecular Weight: 80kDa

Gene ID: 200186

Pathways: AMPK Signaling, Carbohydrate Homeostasis