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Myosin IB antibody (Internal Region)

Verified MYO1B Reactivity: Human, Mouse WB, IHC, ELISA Host: Goat Polyclonal unconjugated
Catalog No. ABIN870635
  • Target See all Myosin IB (MYO1B) Antibodies
    Myosin IB (MYO1B)
    Binding Specificity
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 11
    • 5
    • 4
    Human, Mouse
    Host
    • 13
    • 1
    Goat
    Clonality
    • 14
    Polyclonal
    Conjugate
    • 12
    • 1
    • 1
    This Myosin IB antibody is un-conjugated
    Application
    • 12
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Purpose
    MYO1B
    Sequence
    RTVEAKQEKV STT
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_001123630.1, NP_036355.2). Reported variants represent identical protein: NP_001123630.1, NP_001155291.1.
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-RTVEAKQEKVSTT, from the internal region of the protein sequence according to NP_001123630.1, NP_036355.2.
    Isotype
    IgG
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10 μg/mL.

    Western Blot: Approx 120 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 125 kDa according to Human NP_036355.2 and 128 kDa according to Mouse NP_034993.2). Recommended concentration: 1-3 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Myosin IB (MYO1B)
    Alternative Name
    MYO1B (MYO1B Products)
    Synonyms
    MYO1B antibody, DKEY-242N5.2 antibody, myo1b2 antibody, myo1bl1 antibody, myo1bl2 antibody, si:busm1-60j23.1 antibody, wu:fb39h10 antibody, wu:fc46c09 antibody, zgc:110446 antibody, zgc:113187 antibody, myr1 antibody, AA406997 antibody, myosin-1b antibody, Myr1 antibody, myosin IB antibody, myosin Ib antibody, MYO1B antibody, myo1b antibody, DICPUDRAFT_46917 antibody, Myo1b antibody
    Background
    MYO1B, myosin IB, myr1, MMI-alpha, MMIa, MYH-1c, MYO1B variant protein, OTTHUMP00000165861, myosin I alpha, myosin-I alpha, myosin-Ib
    Molecular Weight
    125kDa
    Gene ID
    4430, 17912, 117057
    NCBI Accession
    NP_001123630, NP_036355
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