CRLS1 antibody (Internal Region)

Catalog No. ABIN870641

The Goat Polyclonal anti-CRLS1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect CRLS1 in samples from Human.

Quick Overview for CRLS1 antibody (Internal Region) (ABIN870641)

Target: CRLS1 (CLS) (Cardiolipin Synthase (CLS))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CRLS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-CRLS1 Antibody

Binding Specificity: Internal Region

Purpose: cardiolipin synthase 1

Sequence: RSALGSALDP LADK

Specificity: This antibody is expected to recognize both reported isoforms (NP_061968.1, NP_001120930.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RSALGSALDPLADK, from the internal region of the protein sequence according to NP_061968.1, NP_001120930.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 30 kDa band observed in lysates of cell line HEK293 (calculated MW of 32.6 kDa according to NP_061968.1). Recommended concentration: 0.2-0.6 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CRLS1

Target: CRLS1 (CLS) (Cardiolipin Synthase (CLS))

Alternative Name: CRLS1

Background: CRLS1, cardiolipin synthase 1, C20orf155, CLS, CLS1, GCD10, dJ967N21.6, cardiolipin synthase

Molecular Weight: 32.6kDa

Gene ID: 54675

NCBI Accession: NP_061968, NP_001120930