EIF2S2 antibody (pSer2)

Catalog No. ABIN871401

The Rabbit Polyclonal anti-EIF2S2 antibody is suitable to detect EIF2S2 in samples from Human and Mouse. It has been validated for WB.

Quick Overview for EIF2S2 antibody (pSer2) (ABIN871401)

Target: EIF2S2 (Eukaryotic Translation Initiation Factor 2, Subunit 2 Beta, 38kDa (EIF2S2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2S2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EIF2S2 Antibody

Binding Specificity: pSer2

Specificity: The antibody detects endogenous levels of eIF2b only when phosphorylated at serine 2.

Purification: Affinity purified

Immunogen: Peptide sequence around phosphorylation site of pSer2 (M-S (p) -G-D-E) derived from eIF2b. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for EIF2S2

Target: EIF2S2 (Eukaryotic Translation Initiation Factor 2, Subunit 2 Beta, 38kDa (EIF2S2))

Alternative Name: eIF2beta

Background: EIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Structure of the beta subunit of translational initiation factor eIF-2. The DNA sequence and comparative analysis of human chromosome 20. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

Molecular Weight: 38 kDa

Gene ID: 8894

NCBI Accession: NP_003899

UniProt: P20042