GAPDH antibody (AA 252-256)
Quick Overview for GAPDH antibody (AA 252-256) (ABIN871714)
Target
See all GAPDH AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 252-256
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Specificity
- The antibody detects endogenous level of total GAPDH protein.
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Purification
- Affinity purified
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Immunogen
- Peptide sequence around AA 252-256 (P-A-K-Y-D) derived from Human GAPDH. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
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anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody
GAPDH Reactivity: Human WB, IF, ICC, IP, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 136-335) antibodyGAPDH Reactivity: Human, Mouse, Rat, Chicken, Monkey, Zebrafish (Danio rerio) WB, IHC, IF, ICC, FACS Host: Rabbit Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibodyGAPDH Reactivity: Human, Mouse, Rat WB, IF, IHC (p) Host: Mouse Monoclonal 1A10A10 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) antibodyVerified GAPDH Reactivity: Human, Rat WB, IHC, ELISA, IF Host: Goat Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 62-91), (N-Term) antibodyGAPDH Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB16537 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) antibodyGAPDH Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) antibodyGAPDH Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3C2 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (Internal Region) antibodyVerified GAPDH Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF Host: Goat Polyclonal unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 233-259), (C-Term) antibodyGAPDH Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16543 unconjugated
anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 3-335) antibodyGAPDH Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
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Application Notes
- Western blotting: 1:500-1:1000
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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Alternative Name
- GAPDH
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Background
- Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
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Molecular Weight
- 37 kDa
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Gene ID
- 2597
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NCBI Accession
- NP_002037
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UniProt
- P04406
Target
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