REC8 antibody (pSer251)

Catalog No. ABIN872285

The Rabbit Polyclonal anti-REC8 antibody has been validated for WB, ELISA, IF (cc), IF (p), IHC (fro) and IHC (p). It is suitable to detect REC8 in samples from Mouse and Rat.

Quick Overview for REC8 antibody (pSer251) (ABIN872285)

Target: REC8 (REC8 Homolog (Yeast) (REC8))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REC8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-REC8 Antibody

Binding Specificity: pSer251

Cross-Reactivity: Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse REC8 around the phosphorylation site of Ser251

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for REC8

Target: REC8 (REC8 Homolog (Yeast) (REC8))

Alternative Name: REC8

Background:

Synonyms: mrec, Rec8L1, Meiotic recombination protein REC8 homolog, Cohesin Rec8p, Rec8, Mei8

Background: Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.

Gene ID: 56739

UniProt: Q8C5S7