VEGFA antibody (AA 81-132)

Catalog No. ABIN872390

The Rabbit Polyclonal anti-VEGFA antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect VEGFA in samples from Human, Rat, Mouse and Sheep. There are 2+ publications available.

Quick Overview for VEGFA antibody (AA 81-132) (ABIN872390)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human, Rat, Mouse, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-VEGFA Antibody

Binding Specificity: AA 81-132

Cross-Reactivity: Human, Mouse, Rat, Sheep

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human VEGF-A

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-VEGFA antibody (ABIN872390)

Li, Li, Sun, Zhou, Li, Feng, Xie: "Dual character of flavonoids in attenuating and aggravating ischemia-reperfusion-induced myocardial injury." in: Experimental and therapeutic medicine, Vol. 14, Issue 2, pp. 1307-1314, (2017) (PubMed).

Gu, Feng, Yu, Yuan, Yin, Ding, Zhao, Xu, Xu, Che: "Fasudil attenuates soluble fms-like tyrosine kinase-1 (sFlt-1)-induced hypertension in pregnant mice through RhoA/ROCK pathway." in: Oncotarget, Vol. 8, Issue 61, pp. 104104-104112, (2017) (PubMed).

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A

Background:

Synonyms: VPF, VEGF, MVCD1, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VEGFA

Background: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Gene ID: 7422

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling