ALOX5AP antibody (AA 65-161)

Catalog No. ABIN872471

The Rabbit Polyclonal anti-ALOX5AP antibody has been validated for WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect ALOX5AP in samples from Rat and Mouse. There is 1 publication available.

Quick Overview for ALOX5AP antibody (AA 65-161) (ABIN872471)

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5AP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ALOX5AP Antibody

Binding Specificity: AA 65-161

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human FLAP/5-lipoxygenase activating protein

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-ALOX5AP antibody (ABIN872471)

Elias, Ferré, Vilà, Muñoz, Casellas, Garcia, Molas, Agudo, Roca, Ruberte, Bosch, Franckhauser: "ALOX5AP Overexpression in Adipose Tissue Leads to LXA4 Production and Protection Against Diet-Induced Obesity and Insulin Resistance." in: Diabetes, Vol. 65, Issue 8, pp. 2139-50, (2016) (PubMed).

Target Details for ALOX5AP

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Alternative Name: 5-lipoxygenase activating protein

Background:

Synonyms: FLAP, Arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein, ALOX5AP

Background: Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.

Gene ID: 241

UniProt: P20292